Signal Transduction Protocols: vol 41 (Methods in Molecular Biology)

• Paperback: 316 pages
• Author: David A. Kendall (Author), Stephen J. Hill (Author)
• Publisher: Humana Press (April 4, 1995)
• Language: English
• ISBN-10: 0896032981
• ISBN-13: 978-0896032989

This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the field as well as those in related areas the opportunity to expand their range of experimental techniques and to carry out successful investigations without needing to refer to any other methodological source.

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Protein Stability and Folding: Theory and Practice: Vol 40 (Methods in Molecular Biology)

• Paperback: 377 pages
• Author: Bret A. Shirley (Author)
• Publisher: Humana Press; 1st edition (May 15, 1995)
• Language: English
• ISBN-10: 0896033015
• ISBN-13: 978-0896033016

In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

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Baculovirus Expression Protocols: Vol 39 (Methods in Molecular Biology)

• Publisher: Humana Press
• Author: Edited by Christopher D Richardson
• Published: 01 January 1995
• Format: eBook 422 pages
• See: Full bibliographic data
• Categories: Medical Microbiology & Virology | Evolution
• ISBN 13: 9786610837366  
• ISBN 10: 6610837368

Over the last 10 years, baculovirus expression vectors have become a very popular and effective means with which to produce recombinant proteins in large quantities. Posttranslational modifications of the gene products of these insect viruses closely parallel glycosylation, fatty acid acylation, and phosphorylation in mammalian cells. Scaleup of insect cells in culture has also been largely perfected, making purification of large quantities of recombinant proteins a reality. 

Cryopreservation and Freeze-Drying Protocols: Vol 38 (Methods in Molecular Biology)

• Paperback: 254 pages
• Author: John G. Day (Editor), Mark R. McLellan (Editor)
• Publisher: Humana Press; 1 edition (March 24, 1995)
• Language: English
• ISBN-10: 0896032965
• ISBN-13: 978-0896032965

This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials.

Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections.

Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.

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In Vitro Transcription and Translation (Methods in Molecular Biology)

• Plastic Comb: 432 pages
• Author: Martin J. Tymms (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1995)
• Language: English
• ISBN-10: 0896032884
• ISBN-13: 978-0896032880

This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques used in the analysis of eukaryotic transcription.

The collection is unique in that it also contains protocols for core techniques that use the products of in vitro transcription and translation systems, such as protein import into mitochondria and chloroplasts and structure-function studies. Also covered are core techniques in the analysis of promoters and transcription factors.

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Peptide Analysis Protocols Vol 36 (Methods in Molecular Biology)

• Plastic Comb: 352 pages
• Author: Ben M. Dunn (Author), Michael W. Pennington (Author)
• Publisher: Humana Press; 1 edition (November 7, 1994)
• Language: English
• ISBN-10: 0896032744
• ISBN-13: 978-0896032743

In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. Chapters also include a helpful Notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments. Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

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Peptide Synthesis Protocols: Vol 35 (Methods in Molecular Biology)

• Paperback: 333 pages
• Author: Michael W. Pennington (Author), Ben M. Dunn (Author)
• Publisher: Humana Press; 1 edition (November 7, 1994)
• Language: English
• ISBN-10: 0896032736
• ISBN-13: 978-0896032736

Immunocytochemical Methods and Protocols: Vol 34 (Methods in Molecular Biology)

• Pub. Date: December 2009
• Publisher: Springer-Verlag New York, LLC
• Series: Methods in Molecular Biology Series
• ISBN-13: 9780896032859
• ISBN: 089603285X

Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocyhemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge prools for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocyhemical techniques in the clinical laboratory and in combination with in situ hybridization.

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In Situ Hybridization Protocols: vol 33 (Methods in Molecular Biology)

• Paperback: 497 pages
• Author: K. H. Andy Choo (Author)
• Publisher: Humana Press (September 1, 1994)
• Language: English
• ISBN-10: 0896032809
• ISBN-13: 978-0896032804

Thirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans.

The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text.

Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.

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Basic Protein and Peptide Protocols: Vol 32 (Methods in Molecular Biology)

• Paperback: 502 pages
• Author: John M. Walker (Editor)
• Publisher: Humana Press; 1 edition (April 26, 1994)
• Language: English
• ISBN-10: 0896032698
• ISBN-13: 978-0896032699

Protocols for Gene Analysis: Vol 31 (Methods in Molecular Biology)

• Paperback: 411 pages
• Author: Adrian J. Harwood (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032582
• ISBN-13: 978-0896032583

This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.

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Chromosome Analysis Protocols: Vol 29 (Methods in Molecular Biology)

• Hardcover: 520 pages
• Author: John R. Gosden (Editor)
• Publisher: Humana Press; 1 edition (February 7, 1994)
• Language: English
• ISBN-10: 0896032892
• ISBN-13: 978-0896032897

Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.

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DNA-Protein Interactions: Principles and Protocols (Methods in Molecular Biology)

• Paperback: 427 pages
• Author: G. Geoff Kneale (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032566
• ISBN-13: 978-0896032569

The clear and easy-to-follow protocols collected here illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal the location of the DNA binding site, the strength and specificity of a binding, the identities of individual groups on the actual bases involved in binding, and the specific amino acid residues of the protein that interact with the DNA. Some of the techniques can even be used to identify previously unknown DNA binding proteins from crude cell extracts, thus empowering you to make groundbreaking advances in your work.

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes: Vol 28 (METHODS IN MOLECULAR BIOLOGY)

• Paperback: 280 pages
• Author: Peter G. Isaac (Author)
• Publisher: Humana Press; 1 edition (November 29, 1993)
• Language: English
• ISBN-10: 089603254X
• ISBN-13: 978-0896032545

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

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Marks' Basic Medical Biochemistry: A Clinical Approach

• Paperback: 900 pages
• Author: Colleen Smith (Author), Allan Marks MD (Author), Michael Lieberman
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• Publisher: Lippincott Williams & Wilkins; Second edition (August 9, 2004)
• Language: English
• ISBN-10: 0781721458
• ISBN-13: 978-0781721455

This best-selling resource utilizes a comprehensive yet practical approach that students understand. The authors present facts and pathways to emphasize how the underlying biochemistry is related to the body's overall physiological functions. Detailed case studies show students the relationship between biochemistry and clinical problems. The Second Edition has been heavily revised with completely updated illustrations while maintaining the unique, patient-oriented approach that made the original so popular.

A new CD-ROM in the back of the book features chapter-by-chapter test questions, detailed 3-D representations of enzyme structure, PowerPoint slides, and real patient stories.

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Instant Notes Biochemistry (Instant Notes (Taylor & Francis))

• Paperback: 422 pages
• Author: B. David Hames (Author), N. M. Hooper (Author)
• Publisher: BIOS Scientific Publishers; 2nd edition (January 15, 2000)
• Language: English
• ISBN-10: 0387916024
• ISBN-13: 978-0387916026

This is a new and up-to-date edition of the best selling "Instant Notes in Biochemistry," providing a valuable revision and information source for all undergraduates taking biochemistry courses. This best-selling Instant Notes book has now been thoroughly updated and reorganized for improved clarity. Many topics have been completely rewritten and new topics on RNA synthesis and processing, and thermodynamics have been added. Remaining concise yet comprehensive, Instant Notes in Biochemistry 2/e continues to provide easy access to the core information required by all students in the life sciences. The format remains ideal for learning and rapid review.

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Interactive Biochemistry CD-ROM

This multimedia tool contains 16 modules, including Bioenergetic Calculations, Biomolecules, Amino Acids, Peptide Bonds, and Protein Structures, Sweet Isomers, Structures of Phospholipids, Nitrogenous Bases, and Nucleosides, Restriction Sites, Enzyme Kinetics and Mechanisms, metabolism and metabolic map Database, and Virtual Biochemical World. Icons integrated throughout Garrett and Grisham's, BIOCHEMISTRY, Second Edition and PRINCIPLES OF BIOCHEMISTRY: A HUMAN FOCUS, First Edition and Campbell's BIOCHEMISTRY, Third Edition prompt students to explore and engage in the activities and exercises provided on the INTERACTIVE BIOCHEMISTRY CD-ROM. Covering every major area of biochemistry, INTERACTIVE BIOCHEMISTRY CD-ROM features 120 Java Applets, 82 Chime™ Tutorials, 8 Virtual Reality scenes, 600 Chime™ structures of Intermediary Metabolites, and interactive problem-solving simulations. The CD-ROM and Workbook are designed to enhance classroom lectures as well as to assist students outside of the classroom. The workbook, which is packaged with the CD-ROM, guides students through the CD modules. Students are asked biochemical questions and they answer the questions in the workbook. The CD-ROM and workbook are value priced when packaged with the text.

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Biomembrane Protocols: II. Architecture and Function: Vol 27 (Methods in Molecular Biology)

• Plastic Comb: 380 pages
• Author: John M. Graham (Author), Joan A. Higgins (Author)
• Publisher: Humana Press; 1 edition (November 28, 1993)
• Language: English
• ISBN-10: 0896032507
• ISBN-13: 978-0896032507

This second volume of protocols offers the most comprehensive set of modern analytical techniques available for studying the architecture and function of membranes. It features the application of biochemical, spectroscopic, and fluorimetric methods to the investigation of molecular topology, the dynamic aspects of membrane structure, and ion transport. Antibody technology, analysis of molecules involved in intracellular signaling, and receptor-ligand interactions are also covered.

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Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques: Vol 26 (Methods in Molecular Biology)

• Paperback: 392 pages
• Author: Sudhir Agrawal (Editor)
• Publisher: Humana Press; 1 edition (November 30, 1993)
• Language: English
• ISBN-10: 0896032523
• ISBN-13: 978-0896032521

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

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Computer Analysis of Sequence Data Part II: Vol 25 (Methods in Molecular Biology)

• Paperback: 433 pages
• Author: Annette M. Griffin, Hugh G. Griffin
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032760
• ISBN-13: 978-0896032767

These comprehensive, up-to-date handbooks are designed for those scientists engaged in the computer analysis of sequence data who want hands-on help in using the most important commercial software available, but simply do not have the time to become computer experts. The expert authors guide you through the programs with easy-to-follow, step-by-step instructions. The topics covered include translations of sequences, sequence alignment, phylogenetic trees, analysis of RNA secondary structure, database searching, submission of data to EMBL/GenBank/DDBJ/etc., maintaining sequence projects, and using patterns to analyze protein sequences. Many chapters have been written by world-class authorities in the field, among them R. Staden, M. Gribskov, D. Higgins, W. Pearson, M. Zuker, and G. Barton. Each volume shares five essential chapters concerning the analysis of sequence data, the FASTA program, converting between sequence formats, obtaining software via INTERNET, and the submission of nucleotide sequence data. Part I covers GCG, MicroGenie, PC/GENE, and FASTA programs. Part II covers Staden and Staden Plus, DNA Strider, FASTA, and MacVector programs.

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Computer Analysis of Sequence Data Part 1: Vol 24 (Methods in Molecular Biology)

• Paperback: 372 pages
• Author: Annette M. Griffin, Hugh G. Griffin
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032469
• ISBN-13: 978-0896032460

 These comprehensive, up-to-date handbooks are designed for those scientists engaged in the computer analysis of sequence data who want hands-on help in using the most important commercial software available, but simply do not have the time to become computer experts. The expert authors guide you through the programs with easy-to-follow, step-by-step instructions. The topics covered include translations of sequences, sequence alignment, phylogenetic trees, analysis of RNA secondary structure, database searching, submission of data to EMBL/GenBank/DDBJ/etc., maintaining sequence projects, and using patterns to analyze protein sequences. Many chapters have been written by world-class authorities in the field, among them R. Staden, M. Gribskov, D. Higgins, W. Pearson, M. Zuker, and G. Barton. Each volume shares five essential chapters concerning the analysis of sequence data, the FASTA program, converting between sequence formats, obtaining software via INTERNET, and the submission of nucleotide sequence data. Part I covers GCG, MicroGenie, PC/GENE, and FASTA programs. Part II covers Staden and Staden Plus, DNA Strider, FASTA, and MacVector programs.

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DNA Sequencing Protocols: Vol 23 (Methods in Molecular Biology)

• Plastic Comb: 392 pages
• Publisher: Humana Press; 1st edition (January 15, 1993)
• Language: English
• ISBN-10: 0896032485
• ISBN-13: 978-0896032484

DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.

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Protocols in Molecular Parasitology: Vol 21 (Methods in Molecular Biology)

• Paperback: 484 pages
• Author: John E. Hyde
• Publisher: Humana Press; 1 edition (August 11, 1993)
• Language: English
• ISBN-10: 0896032396
• ISBN-13: 978-0896032392

Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.

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Protocols for Oligonucleotides and Analogs: Synthesis and Properties: Vol 20 (Methods in Molecular Biology)

• Paperback: 516 pages
• Author: Sudhir Agrawal
• Publisher: Humana Press; 1 edition (December 1, 1993)
• Language: English
• ISBN-10: 0896032477
• ISBN-13: 978-0896032477

Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.

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Biomembrane Protocols: I. Isolation and Analysis: Vol 19 (Methods in Molecular Biology)

• Plastic Comb: 324 pages
• Author: John M. Graham, Joan A. Higgins
• Publisher: Humana Press; 1 edition (August 20, 1993)
• Language: English
• ISBN-10: 0896032361
• ISBN-13: 978-0896032361

Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the isolation and compositional analysis of membranes and address animal, plant, and microbial cells. The topics covered in this first volume include the isolation of subcellular fractions, centrifugation, the extraction and quantitation of lipids, the separation and analysis of proteins, and the isolation and characterization of glycosylated molecules, glycoproteins, proteoglycans, and glycolipids.

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Transgenesis Techniques: Vol. 018 (Methods in Molecular Biology)

• Author: David Murphy, David A. (David Allan) Carter
• Publisher: Humana Press
• Year: 1993 
• Pages: 467 pages

Practical methods for the generation and analysis of transgenic animals, covering a comprehensive range of species, from invertebrates to lower vertebrates to higher vertebrates. Chapters examine ways in which transgenic techniques can be used to address many problems in biological systems present in-depth descriptions of analytical methods, and give step-by-step techniques that can be easily performed in your lab.

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Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques

• Editors: Christopher Jones, Barbara Mulloy, Adrian H. Thomas

• ISBN: 978-0-89603-215-6
• Publisher: Hamana Press. Totowa, New Jersey  

Newton, R. P. (1994), Methods in molecular biology, volume 17: Spectroscopic Methods and Analyses NMR, Mass Spectrometry, and Metalloprotein Techniques EDITED BY C. JONES, B. MULLOY AND A. H. THOMAS Published by Hamana Press. Totowa, New Jersey, 1993 ISBN 0896032159 Price £53.00. Rapid Commun. Mass Spectrom., 8: 224. doi: 10.1002/rcm. 1290080220 

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Enzymes of Molecular Biology: Vol 16 (Methods in Molecular Biology)

• Paperback: 380 pages
• Publisher: Humana Press (June 1, 1993)
• Language: English
• ISBN-10: 0896032345
• ISBN-13: 978-0896032347

• Author: Michael M. Burrell

Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols.

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PCR Protocols: Current Methods and Applications: Vol 15 (Methods in Molecular Biology)

• Paperback: 392 pages
• Author: Bruce A. White
• Publisher: Humana Press (January 1, 1993)
• Language: English
• ISBN-10: 0896032442
• ISBN-13: 978-0896032446

The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols.

Key Topics and Techniques: basic startup procedures for PCR • PCR in detecting DNA and RNA • PCR in DNA synthesis and mutagenesis • PCR cloning of DNA • selection of primers • radioactive and nonradioactive labeling • quantitation of PCR products by HPLC • chromosome assignment • PCR mapping of HLA genes • sequencing of PCR products • genomic footprinting • quantitation of tumor gene expression • screening of phage libraries • SSP-PCR and genome walking • subcloning of PCR products.

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Glycoprotein Analysis in Biomedicine: Vol. 14 (Methods in Molecular Biology) Spi Edition

• Author: Hounsell, Elizabeth F.
• Paperback: 318 pages
• Publisher: Humana Press; Spi edition (January 1, 1993)
• Language: English
• ISBN-10: 0896032264
• ISBN-13: 978-0896032262

Glycoprotein Analysis in Biomedicine brings together a comprehensive range of protocols related to carbohydrate biochemistry. The first half of the manual outlines physiochemical (MS and NMR), chemical, and chromatographic techniques for defining the structures and diversity of oligosaccharide sequences. The second half describes more biological and immunological approaches used to detect changes in glycosylation patterns in disease.

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Protocols in Molecular Neurobiology: Vol. 13 (Methods in Molecular Biology)

• File Size: 23 MB
• Print Length: 394 pages
• Author: Alan Longstaff, Patricia Revest
• Publisher: Humana Press; 1 edition (December 17, 1992)
• Sold by: Amazon Digital Services, Inc.
• Language: English
• ASIN: B001G0MFRY

 Each chapter identifies a problem in neurobiology, showing how the solution of that problem will advance knowledge in the field. The approaches taken to solve the problem are clearly explained, providing sufficient detail for users to readily reproduce the technique and its results. In keeping with the goal of the series, each technique is designed to work, even the first time it is used.

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Pulsed-field Gel Electrophoresis: PROTOCOLS, METHODS & THEORIES Vol 12 (Methods in Molecular Biology)

• Hardcover: 496 pages
• Author: Margit Burmeister, Levy Ulanovsky
• Publisher: Humana Press; 1 edition (January 1, 1991)
• Language: English
• ISBN-10: 0896032299
• ISBN-13: 978-0896032293

Burmeister and Ulanovsky's new volume on Pulsed-Field Gel Electrophoresis presents step-by-step protocols for all users of this powerful methodology, whether novice or expert. The book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed, many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections, which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: • FIGE, CHEF, OFAGE, TAFE, FORE, ODPFGE • preparation of DNA suitable for PFGE from: bacteria, protozoa, plants, mammals, and yeasts. • preparation and analysis of yeast artificial chromosomes (YACS) • mapping strategies • biological applications • theories and observations. In keeping with the well-known practicality of Humana's Methods in Molecular Biology series, Pulsed-Field Gel Electrophoresis brims with protocols designed to work even the first time they are used.

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Practical Protein Chromatography: Vol 11 (Methods in Molecular Biology)

• File Size: 18M
• Author: Andrew Kenney, Susan Fowell
• Print Length: 327 pages
• Publisher: Humana Press; 1 edition (January 15, 1992)
• Language: English
• ISBN-10: 0896032132
• ISBN-13: 978-0896032132

Kenney and Fowell's exceptionally useful new book, Practical Protein Chromatography, brings all the forefront methods of protein separation and purification directly to your laboratory benchtop. The book treats not only powerful analytical separations, but also the small- and large-scale preparative techniques that are currently applicable throughout biological and biomedical research. Chapters, written by experts in each of the techniques covered, treat a variety of methods usable in virtually every laboratory with a chromatographic system. Topics include: immunoaffinity chromatography and selection of antibodies • lectin affinity and dye-ligand chromatography • exploiting weak affinities • alternative coupling chemistries • biospecific affinity elution • size-exclusion HPLC of proteins • chromatofocusing • determination of purity and yield • specialized chromatographic techniques. The established tradition of Methods in Molecular Biology is to present techniques that guarantee optimum results. Everyone doing protein chromatography today-at any level of proficiency-will find Practical Protein Chromatography an indispensable, hands-on guide to the effective use of these methodologies.

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Molecular Bacteriology: Protocols and Clinical Applications

• Hardcover: 682 pages
• Author: by Neil Woodford (Editor), Alan Johnson (Editor)
• Publisher: Humana Press; 1st edition (June 15, 1998)
• Language: English
• ISBN-10: 0896034984
• ISBN-13: 978-0896034983

In Molecular Bacteriology: Protocols and Clinical Applications, Woodford and Johnson bring together in a single volume a state-of-the-art collection of detailed molecular methods for the diagnosis and clinical investigation of bacterial infections. These wide ranging techniques-each presented by recognized experts who have fine-tuned their methods for optimum performance-will permit readers to establish them successfully in their own laboratories. An accompanying series of minireviews highlights and explains the clinical and epidemiological relevance of the techniques and shows how they may be applied to major pathogen species and various types of antibiotic resistance. Molecular Bacteriology: Protocols and Clinical Applications enlarges our current understanding of the role that molecular methodology can play in modern medical bacteriology, fully explaining both the practical and theoretical aspects of the cutting-edge methods that are increasingly finding diagnostic use in laboratories throughout the world. An authoritative laboratory resource for everyone working with infectious diseases today, this book's insightful reviews and detailed protocols will soon win many devotees.

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Roach's Introductory Clinical Pharmacology

• Paperback: 784 pages
• Author: Susan M. Ford, Sally S. Roach
• Publisher: Lippincott Williams & Wilkins; Ninth edition (October 14, 2009)
• Language: English
• ISBN-10: 1605476331
• ISBN-13: 978-1605476339

This core text offers LPN/LVN students a clear, concise introduction to pharmacology, focusing on basic principles and the nurse's responsibility in drug administration. Organized by body system, the book examines pharmacologic properties and therapeutic applications of drug classes. Summary Drug Tables present generic and trade drug names, uses, adverse reactions, and usual dosage ranges. This edition has thoroughly updated drug information, a new "Pharmacology in Practice" case study feature, Nursing Alerts, Lifespan Alerts, Chronic Care Alerts, Diversity Alerts, and additional material on the nursing process.

Including a FREE copy of Lippincott’s Photo Atlas of Medication Administration, a bound CD-ROM, a companion website includes an NCLEX® alternate item format tutorial, a Spanish-English audio glossary, monographs on 100 most commonly prescribed drugs, Listen and Learn, Concepts in Action animations, Watch and Learn video clips, and Dosage Calculation Quizzes. The full text is also available online.

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Biotechnology: Economic and Social Aspects: Issues for Developing Countries

• Paperback: 404 pages
• Author: E. J. DaSilva, C. Ratledge, A. Sasson
• Publisher: Cambridge University Press; 1 edition (November 5, 2009)
• Language: English
• ISBN-10: 0521122287
• ISBN-13: 978-0521122283

This book deals with the important issues involved in developing biotechnologically based industries in developing countries. The science and most of the techniques are well established, and it is often possible to obtain the desired finance. This book however, examines the sorts of choices that a developing country has to make to go ahead with projects and their likely socio-economic consequences. Each of the chapters in this book is written by experts in their field and discusses the current biotechnologically-based industries and their state of development, their suitability for various economies, and the problems associated with developing them. Other chapters discuss environmental questions and further socio-economic factors that need to be considered in order to bring about successful wealth creation in these countries.

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A Course in Monetary Economics: Sequential Trade, Money, and Uncertainity

• Paperback: 424 pages
• Author: Benjamin Eden
• Publisher: Wiley-Blackwell (October 11, 2004)
• Language: English
• ISBN-10: 0631215662
• ISBN-13: 978-0631215660

Monetary Economics and Sequential Trade is an insightful introduction to the advanced topics in monetary economics. Accessible to students who have mastered the diagrammatic tools of economics, it discusses real issues with a variety of modeling alternatives, allowing for a direct comparison of the implications of the different models. The exposition is clear and logical, providing a solid foundation in monetary theory and the techniques of economic modeling. The text is rooted in the author's years of teaching and research, and will be highly suitable for monetary economics courses in both the upper-level undergraduate and graduate levels.

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Economic and Social Issues in Agricultural Biotechnology

• Hardcover: 448 pages
• Author: Robert E Evenson, Vittorio Santaniello, David Zilberman
• Publisher: CABI; First edition (January 7, 2002)
• Language: English
• ISBN-10: 0851996183
• ISBN-13: 978-0851996189

There are currently many controversial socioeconomic issues concerned with the development and implementation of agricultural biotechnology. This book presents selected revised and edited papers from the fourth and fifth meetings of the International Consortium on Agricultural Biotechnology Research, held in Italy in 2000 and 2001.

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Immunochemical Protocols: Vol. 10 (Methods in Molecular Biology)

• Hardcover: 480 pages
• Author: Margaret M. Manson
• Publisher: Humana Press (10 April 1992)
• Language: English
• ISBN-10: 0896032043
• ISBN-13: 978-0896032040

Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.

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Protocols in Human Molecular Genetics: Vol.9 (Methods in Molecular Biology)

• Hardcover: 472 pages
• Author: Mathew
• Publisher: Humana Press Inc. (July 1991)
• Language: English
• ISBN-10: 0896032051
• ISBN-13: 978-0896032057

Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular Biology series. Nearly two-thirds of the book is devoted to describing practical procedures comprising the widest range of new methodologies in human molecular genetics, with the rest focusing on their specific experimental and clinical applications.
An essential tool for everyone-whether novice or seasoned expert-involved in the rapidly growing area of human genome studies.

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Practical Molecular Virology: Viral Vectors for Gene Expression: Vol. 8 (Methods in Molecular Biology)

• Paperback: 330 pages
• Author: Mary K. Collins
• Publisher: Humana Press (31 Oct 1991)
• Language: English
• ISBN-10: 089603299X
• ISBN-13: 978-0896032996

Mary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors. In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: • life cycles of specific retroviruses and how recombinant vectors are constructed • PCR techniques • poliovirus vectors • herpesvirus vectors • syncytial assays • cell lineage studies • baculovirus and adenovirus vectors • SV40 and EBV vectors • viruses in gene transfer to eukaryotic cells The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research.

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Gene Transfer and Expression Protocols: Vol. 7 (Methods in Molecular Biology)

• Hardcover: 440 pages
• Author: Edward J. Murray
• Publisher: Humana Press (22 April 1991)
• Language: English
• ISBN-10: 0896031780
• ISBN-13: 978-0896031784

Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results. This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include: viral vectors • reporter genes • analysis of steady-state level of transcription • assay for newly initiated transcriptional complexes • immunocytological techniques • assay of structure and replication state of transfected genes • overall strategy for defining regulatory sequences Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.

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Plant Cell and Tissue Culture: Vol. 6 (Methods in Molecular Biology)

• Series: Methods in Molecular Biology, Vol. 6
• Author: Pollard, Jeffrey W.; Walker, John M. (Eds.)
  
• Book: 1990, XII, 598 p.
• Publisher: A product of Humana Press
• ISBN: 978-0-89603-161-6

Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells.
Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products.
Chapters cover a wide range of topics and techniques, including:• tissue culture media and selection • cryopreservation • callus culture techniques • organ culture • embryogenesis • batch culture • large-scale culture • hormonal control • fertilization techniques • gene transfer • cell immobilization • production systems • cell product purification • DNA expression • electrofusion of plant cells • mutant selection • mutagenesis techniques • automation • transfer of nuclei • protoplast culture • media analysis • micropropagation.
A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.

 

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Animal Cell Culture: Vol.5 (Methods in Molecular Biology)

• Hardcover: 714 pages
• Author: Jeffrey W. Pollard, John M. Walker
• Publisher: Humana Press; illustrated edition edition (30 Jan 1990)
• Language: English
• ISBN-10: 0896031500
• ISBN-13: 978-0896031500

Animal Cell Culture, the latest volume in Humana's highly successful Methods in Molecular Biology series, provides detailed practical techniques for the culture of a broad spectrum of basic cell cell types. Chapters offer hands-on methods for creating mammalian fibroblastic cell cultures and maintaining culture conditions for epithelial, neuronal, and hematopoietic cells among others. Attention is given to the diversity of culture media and extracellular matrices needed to maintain the differentiated functions of the cultured cells. The book's special strength lies in its descriptions of culture techniques for both living and fixed cells. Chapters cover techniques such as: • cinematographic analysis • in situ mRNA hybridization • immunofluorescence • immunoelectron microscopy • somatic cell hybridization • DNA transformation • insect cell culture • creation of hybridoma cell lines • monoclonal antibody techniques • new, specialized methodologies. A useful appendix lists the most commonly used culture media. Comprehensive in scope and coverage, and thoroughly up-to-date, Pollard and Walker's unique handbook on ANIMAL CELL CULTURE is an indispensable resource for both the novice and the seasoned expert.

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New Nucleic Acid Techniques: Vol.4 (Methods in Molecular Biology)

• Hardcover: 560 pages
• Author: John M. Walker
• Publisher: Humana Press (31 Aug 1988)
• Language: English
• ISBN-10: 0896031276
• ISBN-13: 978-0896031272

Nucleic acids are large biological molecules essential for all known forms of life. They include DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). Together with proteins, nucleic acids are the most important biological macromolecules; each is found in abundance in all living things, where they function in encoding, transmitting and expressing genetic information.

Nucleic acids were discovered by Friedrich Miescher in 1869. Experimental studies of nucleic acids constitute a major part of modern biological and medical research, and form a foundation for genome and forensic science, as well as the biotechnology and pharmaceutical industries.
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New Protein Techniques: Vol.3 (Methods in Molecular Biology)

• Hardcover: 532 pages
• Author: Walker, John M.
• Publisher: Humana Press; illustrated edition edition (30 Aug 1988)
• Language: English
• ISBN-10: 0896031268
• ISBN-13: 978-0896031265

As some of the most commonly studied biological molecules, the activities and structures of proteins are examined both in vitro and in vivo. In vitro studies of purified proteins in controlled environments are useful for learning how a protein carries out its function: for example, enzyme kinetics studies explore the chemical mechanism of an enzyme's catalytic activity and its relative affinity for various possible substrate molecules. By contrast, in vivo experiments on proteins' activities within cells or even within whole organisms can provide complementary information about where a protein functions and how it is regulated.

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Nucleic Acids: Vol.2 (Methods in Molecular Biology)

• Hardcover: 376 pages
• Author: John M. Walker
• Publisher: Humana Press (11 Dec 1984)
• Language: English
• ISBN-10: 0896030644
• ISBN-13: 978-0896030640

 A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyTM series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab

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Proteins (Methods in Molecular Biology Vol. 001)

• Hardcover: 378 pages
• Publisher: Humana Press; 1 edition (November 7, 1984)
• Language: English
• ISBN-10: 0896030628
• ISBN-13: 978-0896030626

The most accurate methods of determining of protein concentration is probably acid hydrolysis followed by amino acid hydrolysis. Most other methods are sensitive to the amino acid concentration of the protein and absolute concentration can not be obtained. This book provided various methods to solve these problems.

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Qualitative Research: Studying How Things Work

• Paperback: 244 pages
• Publisher: The Guilford Press; 1 edition (March 3, 2010)
• Language: English
• ISBN-10: 1606235451
• ISBN-13: 978-1606235454

This book provides invaluable guidance for thinking through and planning a qualitative study. Rather than offering recipes for specific techniques, master storyteller Robert Stake stimulates readers to discover "how things work" in organizations, programs, communities, and other systems. Topics range from identifying a research question to selecting methods, gathering data, interpreting and analyzing the results, and producing a well-thought-through written report. In-depth examples from actual studies emphasize the role of the researcher as instrument and interpreter, while boxed vignettes and learning projects encourage self-reflection and critical thinking. Other useful pedagogical features include quick-reference tables and charts, sample project management forms, and an end-of-book glossary. After reading this book, doctoral students and novice qualitative researchers will be able to plan a study from beginning to end.

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