- Paperback: 280 pages
- Author: Peter G. Isaac (Author)
- Publisher: Humana Press; 1 edition (November 29, 1993)
- Language: English
- ISBN-10: 089603254X
- ISBN-13: 978-0896032545
This book provides an exceptionally useful collection of tried and
tested protocols for nucleic acid analysis that avoid the use of
radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting
protocols are covered in detail, from how to isolate good quality
nucleic acids from plant and animal sources, to the use of the widest
range of probe systems (involving digoxigenin, biotin, fluorescein,
horseradish peroxidase labeling, and colorimetric and chemiluminescent
detection). Other techniques treated in detail include hybridization in
situ to chromosomes and transcribed RNA, newer and less conventional
technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse
dot blots, bacterial identification using magnetic bead and dipstick
assays, and nucleic acid sequence-based amplification.
Link (Rapidshare.com)
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