Signal Transduction Protocols: vol 41 (Methods in Molecular Biology)

• Paperback: 316 pages
• Author: David A. Kendall (Author), Stephen J. Hill (Author)
• Publisher: Humana Press (April 4, 1995)
• Language: English
• ISBN-10: 0896032981
• ISBN-13: 978-0896032989

This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the field as well as those in related areas the opportunity to expand their range of experimental techniques and to carry out successful investigations without needing to refer to any other methodological source.

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Protein Stability and Folding: Theory and Practice: Vol 40 (Methods in Molecular Biology)

• Paperback: 377 pages
• Author: Bret A. Shirley (Author)
• Publisher: Humana Press; 1st edition (May 15, 1995)
• Language: English
• ISBN-10: 0896033015
• ISBN-13: 978-0896033016

In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

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Baculovirus Expression Protocols: Vol 39 (Methods in Molecular Biology)

• Publisher: Humana Press
• Author: Edited by Christopher D Richardson
• Published: 01 January 1995
• Format: eBook 422 pages
• See: Full bibliographic data
• Categories: Medical Microbiology & Virology | Evolution
• ISBN 13: 9786610837366  
• ISBN 10: 6610837368

Over the last 10 years, baculovirus expression vectors have become a very popular and effective means with which to produce recombinant proteins in large quantities. Posttranslational modifications of the gene products of these insect viruses closely parallel glycosylation, fatty acid acylation, and phosphorylation in mammalian cells. Scaleup of insect cells in culture has also been largely perfected, making purification of large quantities of recombinant proteins a reality. 

Cryopreservation and Freeze-Drying Protocols: Vol 38 (Methods in Molecular Biology)

• Paperback: 254 pages
• Author: John G. Day (Editor), Mark R. McLellan (Editor)
• Publisher: Humana Press; 1 edition (March 24, 1995)
• Language: English
• ISBN-10: 0896032965
• ISBN-13: 978-0896032965

This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials.

Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections.

Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.

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In Vitro Transcription and Translation (Methods in Molecular Biology)

• Plastic Comb: 432 pages
• Author: Martin J. Tymms (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1995)
• Language: English
• ISBN-10: 0896032884
• ISBN-13: 978-0896032880

This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques used in the analysis of eukaryotic transcription.

The collection is unique in that it also contains protocols for core techniques that use the products of in vitro transcription and translation systems, such as protein import into mitochondria and chloroplasts and structure-function studies. Also covered are core techniques in the analysis of promoters and transcription factors.

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Peptide Analysis Protocols Vol 36 (Methods in Molecular Biology)

• Plastic Comb: 352 pages
• Author: Ben M. Dunn (Author), Michael W. Pennington (Author)
• Publisher: Humana Press; 1 edition (November 7, 1994)
• Language: English
• ISBN-10: 0896032744
• ISBN-13: 978-0896032743

In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. Chapters also include a helpful Notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments. Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

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Peptide Synthesis Protocols: Vol 35 (Methods in Molecular Biology)

• Paperback: 333 pages
• Author: Michael W. Pennington (Author), Ben M. Dunn (Author)
• Publisher: Humana Press; 1 edition (November 7, 1994)
• Language: English
• ISBN-10: 0896032736
• ISBN-13: 978-0896032736

Immunocytochemical Methods and Protocols: Vol 34 (Methods in Molecular Biology)

• Pub. Date: December 2009
• Publisher: Springer-Verlag New York, LLC
• Series: Methods in Molecular Biology Series
• ISBN-13: 9780896032859
• ISBN: 089603285X

Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocyhemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge prools for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocyhemical techniques in the clinical laboratory and in combination with in situ hybridization.

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