In Situ Hybridization Protocols: vol 33 (Methods in Molecular Biology)

• Paperback: 497 pages
• Author: K. H. Andy Choo (Author)
• Publisher: Humana Press (September 1, 1994)
• Language: English
• ISBN-10: 0896032809
• ISBN-13: 978-0896032804

Thirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans.

The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text.

Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.

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Basic Protein and Peptide Protocols: Vol 32 (Methods in Molecular Biology)

• Paperback: 502 pages
• Author: John M. Walker (Editor)
• Publisher: Humana Press; 1 edition (April 26, 1994)
• Language: English
• ISBN-10: 0896032698
• ISBN-13: 978-0896032699

Protocols for Gene Analysis: Vol 31 (Methods in Molecular Biology)

• Paperback: 411 pages
• Author: Adrian J. Harwood (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032582
• ISBN-13: 978-0896032583

This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.

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Chromosome Analysis Protocols: Vol 29 (Methods in Molecular Biology)

• Hardcover: 520 pages
• Author: John R. Gosden (Editor)
• Publisher: Humana Press; 1 edition (February 7, 1994)
• Language: English
• ISBN-10: 0896032892
• ISBN-13: 978-0896032897

Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.

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DNA-Protein Interactions: Principles and Protocols (Methods in Molecular Biology)

• Paperback: 427 pages
• Author: G. Geoff Kneale (Editor)
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032566
• ISBN-13: 978-0896032569

The clear and easy-to-follow protocols collected here illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal the location of the DNA binding site, the strength and specificity of a binding, the identities of individual groups on the actual bases involved in binding, and the specific amino acid residues of the protein that interact with the DNA. Some of the techniques can even be used to identify previously unknown DNA binding proteins from crude cell extracts, thus empowering you to make groundbreaking advances in your work.

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes: Vol 28 (METHODS IN MOLECULAR BIOLOGY)

• Paperback: 280 pages
• Author: Peter G. Isaac (Author)
• Publisher: Humana Press; 1 edition (November 29, 1993)
• Language: English
• ISBN-10: 089603254X
• ISBN-13: 978-0896032545

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

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Marks' Basic Medical Biochemistry: A Clinical Approach

• Paperback: 900 pages
• Author: Colleen Smith (Author), Allan Marks MD (Author), Michael Lieberman
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• Publisher: Lippincott Williams & Wilkins; Second edition (August 9, 2004)
• Language: English
• ISBN-10: 0781721458
• ISBN-13: 978-0781721455

This best-selling resource utilizes a comprehensive yet practical approach that students understand. The authors present facts and pathways to emphasize how the underlying biochemistry is related to the body's overall physiological functions. Detailed case studies show students the relationship between biochemistry and clinical problems. The Second Edition has been heavily revised with completely updated illustrations while maintaining the unique, patient-oriented approach that made the original so popular.

A new CD-ROM in the back of the book features chapter-by-chapter test questions, detailed 3-D representations of enzyme structure, PowerPoint slides, and real patient stories.

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Instant Notes Biochemistry (Instant Notes (Taylor & Francis))

• Paperback: 422 pages
• Author: B. David Hames (Author), N. M. Hooper (Author)
• Publisher: BIOS Scientific Publishers; 2nd edition (January 15, 2000)
• Language: English
• ISBN-10: 0387916024
• ISBN-13: 978-0387916026

This is a new and up-to-date edition of the best selling "Instant Notes in Biochemistry," providing a valuable revision and information source for all undergraduates taking biochemistry courses. This best-selling Instant Notes book has now been thoroughly updated and reorganized for improved clarity. Many topics have been completely rewritten and new topics on RNA synthesis and processing, and thermodynamics have been added. Remaining concise yet comprehensive, Instant Notes in Biochemistry 2/e continues to provide easy access to the core information required by all students in the life sciences. The format remains ideal for learning and rapid review.

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Interactive Biochemistry CD-ROM

This multimedia tool contains 16 modules, including Bioenergetic Calculations, Biomolecules, Amino Acids, Peptide Bonds, and Protein Structures, Sweet Isomers, Structures of Phospholipids, Nitrogenous Bases, and Nucleosides, Restriction Sites, Enzyme Kinetics and Mechanisms, metabolism and metabolic map Database, and Virtual Biochemical World. Icons integrated throughout Garrett and Grisham's, BIOCHEMISTRY, Second Edition and PRINCIPLES OF BIOCHEMISTRY: A HUMAN FOCUS, First Edition and Campbell's BIOCHEMISTRY, Third Edition prompt students to explore and engage in the activities and exercises provided on the INTERACTIVE BIOCHEMISTRY CD-ROM. Covering every major area of biochemistry, INTERACTIVE BIOCHEMISTRY CD-ROM features 120 Java Applets, 82 Chime™ Tutorials, 8 Virtual Reality scenes, 600 Chime™ structures of Intermediary Metabolites, and interactive problem-solving simulations. The CD-ROM and Workbook are designed to enhance classroom lectures as well as to assist students outside of the classroom. The workbook, which is packaged with the CD-ROM, guides students through the CD modules. Students are asked biochemical questions and they answer the questions in the workbook. The CD-ROM and workbook are value priced when packaged with the text.

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Biomembrane Protocols: II. Architecture and Function: Vol 27 (Methods in Molecular Biology)

• Plastic Comb: 380 pages
• Author: John M. Graham (Author), Joan A. Higgins (Author)
• Publisher: Humana Press; 1 edition (November 28, 1993)
• Language: English
• ISBN-10: 0896032507
• ISBN-13: 978-0896032507

This second volume of protocols offers the most comprehensive set of modern analytical techniques available for studying the architecture and function of membranes. It features the application of biochemical, spectroscopic, and fluorimetric methods to the investigation of molecular topology, the dynamic aspects of membrane structure, and ion transport. Antibody technology, analysis of molecules involved in intracellular signaling, and receptor-ligand interactions are also covered.

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Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques: Vol 26 (Methods in Molecular Biology)

• Paperback: 392 pages
• Author: Sudhir Agrawal (Editor)
• Publisher: Humana Press; 1 edition (November 30, 1993)
• Language: English
• ISBN-10: 0896032523
• ISBN-13: 978-0896032521

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

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Computer Analysis of Sequence Data Part II: Vol 25 (Methods in Molecular Biology)

• Paperback: 433 pages
• Author: Annette M. Griffin, Hugh G. Griffin
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032760
• ISBN-13: 978-0896032767

These comprehensive, up-to-date handbooks are designed for those scientists engaged in the computer analysis of sequence data who want hands-on help in using the most important commercial software available, but simply do not have the time to become computer experts. The expert authors guide you through the programs with easy-to-follow, step-by-step instructions. The topics covered include translations of sequences, sequence alignment, phylogenetic trees, analysis of RNA secondary structure, database searching, submission of data to EMBL/GenBank/DDBJ/etc., maintaining sequence projects, and using patterns to analyze protein sequences. Many chapters have been written by world-class authorities in the field, among them R. Staden, M. Gribskov, D. Higgins, W. Pearson, M. Zuker, and G. Barton. Each volume shares five essential chapters concerning the analysis of sequence data, the FASTA program, converting between sequence formats, obtaining software via INTERNET, and the submission of nucleotide sequence data. Part I covers GCG, MicroGenie, PC/GENE, and FASTA programs. Part II covers Staden and Staden Plus, DNA Strider, FASTA, and MacVector programs.

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Computer Analysis of Sequence Data Part 1: Vol 24 (Methods in Molecular Biology)

• Paperback: 372 pages
• Author: Annette M. Griffin, Hugh G. Griffin
• Publisher: Humana Press; 1st edition (January 15, 1994)
• Language: English
• ISBN-10: 0896032469
• ISBN-13: 978-0896032460

 These comprehensive, up-to-date handbooks are designed for those scientists engaged in the computer analysis of sequence data who want hands-on help in using the most important commercial software available, but simply do not have the time to become computer experts. The expert authors guide you through the programs with easy-to-follow, step-by-step instructions. The topics covered include translations of sequences, sequence alignment, phylogenetic trees, analysis of RNA secondary structure, database searching, submission of data to EMBL/GenBank/DDBJ/etc., maintaining sequence projects, and using patterns to analyze protein sequences. Many chapters have been written by world-class authorities in the field, among them R. Staden, M. Gribskov, D. Higgins, W. Pearson, M. Zuker, and G. Barton. Each volume shares five essential chapters concerning the analysis of sequence data, the FASTA program, converting between sequence formats, obtaining software via INTERNET, and the submission of nucleotide sequence data. Part I covers GCG, MicroGenie, PC/GENE, and FASTA programs. Part II covers Staden and Staden Plus, DNA Strider, FASTA, and MacVector programs.

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DNA Sequencing Protocols: Vol 23 (Methods in Molecular Biology)

• Plastic Comb: 392 pages
• Publisher: Humana Press; 1st edition (January 15, 1993)
• Language: English
• ISBN-10: 0896032485
• ISBN-13: 978-0896032484

DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.

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Protocols in Molecular Parasitology: Vol 21 (Methods in Molecular Biology)

• Paperback: 484 pages
• Author: John E. Hyde
• Publisher: Humana Press; 1 edition (August 11, 1993)
• Language: English
• ISBN-10: 0896032396
• ISBN-13: 978-0896032392

Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.

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Protocols for Oligonucleotides and Analogs: Synthesis and Properties: Vol 20 (Methods in Molecular Biology)

• Paperback: 516 pages
• Author: Sudhir Agrawal
• Publisher: Humana Press; 1 edition (December 1, 1993)
• Language: English
• ISBN-10: 0896032477
• ISBN-13: 978-0896032477

Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.

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Biomembrane Protocols: I. Isolation and Analysis: Vol 19 (Methods in Molecular Biology)

• Plastic Comb: 324 pages
• Author: John M. Graham, Joan A. Higgins
• Publisher: Humana Press; 1 edition (August 20, 1993)
• Language: English
• ISBN-10: 0896032361
• ISBN-13: 978-0896032361

Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the isolation and compositional analysis of membranes and address animal, plant, and microbial cells. The topics covered in this first volume include the isolation of subcellular fractions, centrifugation, the extraction and quantitation of lipids, the separation and analysis of proteins, and the isolation and characterization of glycosylated molecules, glycoproteins, proteoglycans, and glycolipids.

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